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WormClassroom.org: An Inquiry-rich Educational Web Portal for Research Resources of Caenorhabditis elegans

Fong-Mei Lu, Kevin W. Eliceiri^*, James Stewart, and John G. White^*

*Laboratory for Optical and Computational Instrumentation, Laboratory of Molecular Biology, and Department of Curriculum and Instruction, School of Education, University of Wisconsin-Madison, Madison, WI 53706

Submitted July 31, 2006; Revised November 28, 2006; Accepted December 28, 2006

Monitoring Editor: John Jungck

	ABSTRACT

TOP ABSTRACT INTRODUCTION C. elegans: THE ORGANISM... THE EDUCATIONAL PORTAL: THE... CONCLUSION AND DISCUSSION REFERENCES

 
The utilization of biology research resources, coupled with a "learning by inquiry" approach, has great potential to aid students in gaining an understanding of fundamental biological principles. To help realize this potential, we have developed a Web portal for undergraduate biology education, WormClassroom.org, based on current research resources of a model research organism, Caenorhabditis elegans. This portal is intended to serve as a resource gateway for students to learn biological concepts using C. elegans research material. The driving forces behind the WormClassroom website were the strengths of C. elegans as a teaching organism, getting researchers and educators to work together to develop instructional materials, and the 3 P's (problem posing, problem solving, and peer persuasion) approach for inquiry learning. Iterative assessment is an important aspect of the WormClassroom site development because it not only ensures that content is up-to-date and accurate, but also verifies that it does, in fact, aid student learning. A primary assessment was performed to refine the WormClassroom website utilizing undergraduate biology students and nonstudent experts such as C. elegans researchers; results and comments were used for site improvement. We are actively encouraging continued resource contributions from the C. elegans research and education community for the further development of WormClassroom.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION C. elegans: THE ORGANISM... THE EDUCATIONAL PORTAL: THE... CONCLUSION AND DISCUSSION REFERENCES

 
Current education reform efforts have called for a balance of teaching and research in the university environment (Chalupa, 1999; Cech, 2003). These calls signify the recognition of the disproportionate emphasis on research over teaching at many major universities and the need to give a higher priority to undergraduate education. There have been several significant efforts to try to achieve a better balance between research and teaching in academia (Avila, 2003; Cech, 2003; Wood and Gentile, 2003). One of the most prominent is the funding of teacher-scholars by the Howard Hughes Medical Institute (HHMI) to develop new modes of science teaching (HHMI, 2002, 2006). The funding of HHMI teacher-scholars was a significant new venture for a research foundation best known for supporting excellence in research. One of the early innovations of this HHMI teacher-scholar effort is the "incubator" concept developed by Handelsman et al. (2004). In the article "scientific teaching," Handelsman et al. (2004) have urged universities to promote education reform by supporting the development of incubators "... where researchers incorporate research results into teaching materials with guidance from experts in pedagogy." This incubator concept highlights three important aspects of current reform efforts: 1) the need for researchers and educators to work together, 2) the utilization of research resources to promote student learning, and 3) the training of future faculty to develop teaching materials that take student learning into account. This approach is intriguing because education reform efforts need attention from both educators and science researchers. Bringing together researchers and educators has the key advantage that the compiled resources are accurate, up-to-date, and student-learning driven. Finally, there is the additional benefit that research data are generated from actual research lab problems and investigations that are of interest to students.

We were excited by the incubator concept because, like many research groups, we had informally applied research resources from our own lab to biology education. Our utilization of research resources to education was driven by the recognition that students learn better by active engagement in the process of scientific inquiry (National Research Council [NRC], 2000). In particular, we were influenced by the 3 P's (problem posing, problem solving, and peer persuasion) approach developed by BioQUEST (2000) for learning biology, in which the 3 P's are used to engage students in authentic biological inquiry (Peterson and Jungck, 1988). Guided by the 3 P's approach for inquiry learning, we, a group of researchers and educators, began to develop instructional materials (IMs) that utilize resources of a model organism, Caenorhabditis elegans (C. elegans).

C. elegans (often referred to as "the worm"), although relatively primitive, shares many biological properties with more advanced organisms, including humans. It has proved to be a useful model research organism because of its genetic homology with higher organisms as well as similarities in morphological traits and development strategies. Research on C. elegans has shed light on many fundamental biological phenomena, such as cell fate determination and programmed cell death, that have important implications for human health.

Despite its popularity in research, the use of C. elegans and its research resources in the classroom are not widespread. One obstacle to its educational use may be that these research resources are primarily targeted for professionals in the research community, making it difficult for those outside the field to utilize them in undergraduate education. As a result, the use of C. elegans in the classroom is often limited to instructors who have C. elegans research backgrounds. Rectifying this lack of accessibility requires that researchers and educators work together in order to make the resources available for educational purposes.

Arising initially out of a desire within our laboratory to utilize research resources related to C. elegans cell division for educational purposes, we developed an education website, WormClassroom (http://www.WormClassroom.org). Through collaborations with other C. elegans labs, we came to realize the potential for a C. elegans community educational Web portal that could organize and link to research resources for inquiry-based learning.

In this article, we will describe the following: I. C. elegans—the organism and its professional community: 1) C. elegans as a valuable teaching organism and 2) educators and C. elegans professionals who have been involved in our efforts for making the WormClassroom site possible. II. The educational portal—the WormClassroom website: 1) current status of the WormClassroom website, 2) development of IMs utilizing the 3 P's approach, 3) example utilizations for inquiry learning, and 4) assessment of the WormClassroom site for its improvement and future C. elegans research resource applications to education.

	C. elegans: THE ORGANISM AND ITS PROFESSIONAL COMMUNITY

TOP ABSTRACT INTRODUCTION C. elegans: THE ORGANISM... THE EDUCATIONAL PORTAL: THE... CONCLUSION AND DISCUSSION REFERENCES

 
C. elegans as a Valuable Teaching Organism

Caenorhabditis elegans (Caeno, recent; rhabditis, rod; elegans, nice) was first introduced by Sydney Brenner as a model organism for pursuing research in developmental biology and neurology in the 1960s. It is a free-living, nonparasitic soil nematode that can be safely used in the laboratory and is common around the world (Donald, 1997). It is small (1 mm in length), transparent, and easily manipulated and observed; feeds on bacteria (such as Escherichia coli); and can be easily and cheaply cultivated in large numbers (10,000 worms per Petri dish) in the laboratory. It has a short life cycle: one generation takes 3 d. Its life span is about 2–3 wk under suitable living conditions. What is unique to this organism is that wild-type individuals contain a constant 959 cells. The position of these cells is also constant. Because the worm is transparent, it is possible to track cells and follow cell lineages. The complete cell lineage of C. elegans was completed in the 1980s by John Sulston. Moreover, it was the first multicellular organism to have its genome completely sequenced (December 1998). This combination of attributes makes C. elegans an ideal tool for research.

The use of this small worm in educational settings has grown in recent years. At the 15th International C. elegans Meeting, held at the University of California at Los Angeles in 2005, 14 teaching posters were presented, as well as an education panel focusing on the use of C. elegans in higher education. C. elegans is used in various disciplines to convey fundamental biological concepts. For instance, it is a good organism for students to observe animal behavior and on which to perform phenotype analyses. In addition, the available research resources, such as the stock of mutants, the complete genome sequence, and various genetic research tools, make C. elegans a valuable tool in classrooms. C. elegans is a well-established model system with a wealth of research resources that can be utilized in teaching biology.

Educators and C. elegans Professionals

The WormClassroom site owes its development to many C. elegans experts and educators. To date, most of the contributions have originated from the University of Wisconsin (UW)-Madison C. elegans community. However, there have been several key contributions from researchers worldwide. A comprehensive list of current contributors is available at the WormClassroom website (http://www.wormclassroom.org/contacts.html).

	THE EDUCATIONAL PORTAL: THE WormClassroom WEBSITE

TOP ABSTRACT INTRODUCTION C. elegans: THE ORGANISM... THE EDUCATIONAL PORTAL: THE... CONCLUSION AND DISCUSSION REFERENCES

 
Our goal for WormClassroom is to make C. elegans research resources accessible in a form that can be used to develop inquiry-oriented instruction. In developing a Web portal of research data, no matter whether the content is a database, such as WormBase.org, or collection of data, such as CellsAlive.org, the organization of the resources is the primary challenge. The resources need to be organized in a way that provides students an appreciation of C. elegans and associated scientific research while allowing them to inquire using these resources to gain an understanding of basic scientific concepts.

Current Status of the WormClassroom Website

Resource Organization of the WormClassroom Site.

The C. elegans researchers often identify themselves as members of other research communities. As a result, in the WormClassroom site, we categorized the C. elegans resources into five groupings: Cell Biology, Developmental Biology, Genetics, Evolutionary Biology, and Neuroscience. Figure 1 shows the homepage of the WormClassroom. This main entry page serves to present the resources of C. elegans, as well as additional materials to aid in the use of these resources. These additional materials include information on the development of the site, news items, a search query, and a guide to assist in the effective use of WormClassroom.

View larger version (34K): [in this window] [in a new window]   Figure 1. Home page of WormClassroom website.

Resource Organization within the Associated Disciplines.

For resources within each research field, we apply the "consensus practice" developed by Kitcher (1984, 1993) for further resource organization. Frequently, in a research field, scientists have their own ways of solving problems and their own languages to assist their communication during inquiry. Kitcher described this practice as a "consensus practice" among investigators in the same research community who share the following: 1) a common language, 2) a set of questions taken to be most appropriate, 3) defined patterns of reasoning, and 4) a set of experimental procedures and guidelines for investigation. The concept of "consensus practice" provides a good set of guidelines for us to organize resources within a given discipline. This resource organization is used to familiarize students with C. elegans–based inquiry within a particular research field and encourages students to think like researchers. As stated, resources of C. elegans are sorted into five major research disciplines. For the Web interface for each of these five disciplines, we have organized the materials and information in a similar manner to generate the same look and feel between each category. To explain further, we will use the Cell Biology category as an example (see Figure 2, the entry page for Cell Biology). To introduce students to Cell Biology inquiry that utilizes C. elegans, we provide a set of questions often pursued by researchers in the discipline (Cell Biology Research Questions). Examples of research are illustrated, providing students with an in-depth look at C. elegans research in the field of Cell Biology (Example Research). A variety of Cell Biology research using C. elegans is also identified and made accessible for students so that they might understand the breadth of C. elegans research in this field. Research tools and methodologies used in C. elegans research are identified and described in order to provide users with an overview of the process of conducting cell biology research utilizing C. elegans (Cell Biology Research: Tools, Procedures, and Methodologies). Associated teaching materials (IMs) are also available for each research category. Terminology used in the field of Cell Biology using C. elegans is identified and defined (Glossary).

View larger version (32K): [in this window] [in a new window]   Figure 2. Entry page to the C. elegans research in cell biology.

IMs for the WormClassroom Website

We sought ways to develop IMs that combined input from both researchers and educators. Our work was primarily guided by the 3 P's approach (Peterson and Jungck, 1988). The concept of the 3 P's for learning biology was first proposed in the 1980s. To develop materials for inquiry learning, the 3 P's approach guided us in creating a question space to assist students in learning how to pose problems while simultaneously supplying resources and tools for student investigation.

Below, we describe the development of IMs that are available at the WormClassroom website. These materials include 1) raw research data such as movies of embryo development, 2) animations to illustrate key biological phenomena, and 3) learning modules with specific learning objectives identified.

Raw Research Data.

The raw research data includes resources such as images, movies, sequence data of proteins, RNA transcripts, and genes. Because these data resources are traditionally targeted to researchers, a certain amount of background material is needed to help engage students in inquiry learning. To create a question space in which students may pose problems using these data, we provide as much background information as possible, without interpreting the data and the phenomenon shown. For instance, a movie (Figure 3, left image) that depicts a fertilized egg developing into a worm provides students the opportunity to inquire into many fundamental phenomena such as embryonic cleavage. Background resources accompanying this movie include information such as 1) the technical methods utilized for the movie acquisition, such as the type of microscopy used, and 2) data on key attributes of the embryo, such as its dimensions. Annotated images of the embryo are also provided with major components of the embryo labeled (see Figure 3, right image).

View larger version (33K): [in this window] [in a new window]   Figure 3. Raw research data and its accompanied background resource. (A) A differential interference contrast (DIC) movie of C. elegans embryo development (image courtesy of Ananth Badrinath, University of Southern California). (B) An annotated image of a two-cell embryo with nuclei labeled. The dimension of the embryo is 55 x 30 µm.

Animations are valuable educational tools because they depict phenomena that cannot be easily understood using still images (Stith, 2004). We utilized a type of animation called a "BioClip" (BioClips, 2004) that combines animations of a biological process with appropriate background materials. BioClips can be used to create a question space for student observation and for posing problems. For instance, we developed a cell polarity BioClip that illustrates cell polarization of a C. elegans embryo from a one- to four-cell stage (Lu et al., 2007). For the development of the BioClip animation, we were guided by current research data of cell polarization in C. elegans. In the animation, we illustrate the phenomenon without imposing any current scientific interpretations to this animation, such as illustrating the interaction between microtubules and the membrane cortex without depicting the force between them. The animation is displayed next to a movie obtained with videomicroscopy (see Figure 4). Both movies are then made available online for student observation and problem posing. Examples of the types of problems students might pose are discussed in the section, "Utilization of WormClassroom Website for Inquiry Learning."

View larger version (37K): [in this window] [in a new window]   Figure 4. Movies of cell polarity animation with a matching real embryo development. (A) Animation of cell polarization in C. elegans early embryonic development. (B) DIC movie of a C. elegans embryonic development (image courtesy of Haining Zhang, University of Wisconsin-Madison).

We define a learning module as an interactive and self-contained learning environment complete with its own learning objectives that is accessible online. The raw research data and corresponding animations can be integrated into the learning module. Each learning module starts with biological phenomena to serve as a question space and induce student curiosity. Background resources and materials, as well as tools and additional resources, are made available to aid student investigation. For instance, the learning module Cell Lineage allows students to track a cell's development by tracing its predecessors and successors. Students can also investigate the spatial relationship (e.g., left/right) of cells during an embryo's development (http://www.wormclassroom.org/modules/celllineage/). This module opens with a flash animation illustrating the development from a zygote to a complete worm, with its cell types labeled. Along with the development, lines of different colors represent development of different cell types that ultimately form a functional worm. By observing this animation, students may wonder "what happens at branch points that generate daughter cells with different colors?" As shown in Figure 5, the developmental potential of cells in C. elegans is determined early in development (two-cell stage). The module also includes background information, questions to encourage and direct student thinking, and activities such as characterizing and analyzing the C. elegans developmental lineage. Inside each activity, movies of embryos are used for student inquiry into the lineage of cells and development of cells with different developmental potentials.

View larger version (25K): [in this window] [in a new window]   Figure 5. Cell lineage learning module. (A) The home page of the cell lineage module. (B) An annotated image of an embryo at eight-cell embryonic stage. (C) The image in the middle with cells in corresponding colors shown to the left.

Utilization of WormClassroom Website for Inquiry Learning

Materials on the WormClassroom website are designed for curriculum development. Instructors play a major role in specific materials selection to meet their instructional goals. Below we demonstrate the use of resources found in the WormClassroom website to engage students with research data. We will explore how movies of developing embryos, along with their accompanying resources, allow students to inquire into many fundamental biological concepts.

Phenomenon Observation and Background Information.

Figure 6 shows images of five C. elegans embryos after the first embryonic division. (These images are available in the WormClassroom website as movies.) In the classroom, students can be directed to these online movies for observation.

View larger version (29K): [in this window] [in a new window]   Figure 6. Images of five C. elegans embryos after first embryonic division (two-cell stage). (A) Wild-type. (B) Mutant 1. (C) Mutant 2. (D) Mutant 3. (E) Mutant 4 (images courtesy of Kevin O'Connell, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health).

View larger version (31K): [in this window] [in a new window]   Figure 7. Background information about phenomena in observation. (A) Top, an adult C. elegans with scale; bottom, an illustration of C. elegans with its anatomy including embryos and oocytes (image courtesy of WormAtlas.org). (B) An annotated image showing a fertilized egg with its paternal and maternal pronuclei labeled.

At this stage, students will be posing questions regarding their observations. Table 2 presents a list of possible questions. As listed, the questions that might arise from observing the phenomena range from basic to advanced and span the disciplines of genetics, cell biology, and developmental biology.

As discussed, the problems posed could range from basic, such as questions regarding the physical dimensions of the embryo, to advanced, such as genetic causes of the phenomenon shown in Figure 6. To help answer questions regarding the size of the embryo and daughter cells, the movie of the wild-type embryo can be used in conjunction with available measurement tools such as ImageJ (National Institutes of Health, 1999). Information on the movie acquisition provides students the opportunity to understand that the development of an embryo is four-dimensional (4D = 3D + time) and that the embryo itself has dimensionality. A 4D dataset of wild-type embryo development is available for investigating questions regarding size.

Figure 8 depicts a two-cell stage embryo that illustrates how the size of the two changes along the different sections in three dimensions. By making their own drawings and measurements, students may come to understand that a C. elegans embryo is oval, like a chicken egg, and has dimensionality. For example, the change in size of the nuclei is only evident in certain spatial dimensions and varies again over time. Elements or materials of a cell may not be seen either because they are not present or because they are not observable in the current section.

View larger version (6K): [in this window] [in a new window]   Figure 8. Outlines of the wild-type embryo in two-cell embryonic stage. (A) Section 2, neither of the nuclei are seen. (B) Section 4, nucleus on the right is larger than the one on the left. (C) Section 5, two nuclei seem to be the same size. (D) Section 7, nucleus on the right seems to be smaller than the left one.

View larger version (30K): [in this window] [in a new window]   Figure 9. Images of different microscopy in the study of cell division in C. elegans. A C. elegans embryo at pronuclei meeting stage (before dividing into a two-cell embryo). (A) Differential interference contrast (DIC; Nomarski; image courtesy of Haining Zhang, University of Wisconsin-Madison). (B) Laser scanning confocal microscopy (LSCM; image courtesy of Maria Vidal Dinkelmann, University of Wisconsin-Madison). (C) Multiphoton fluorescence excitation microscopy (MPFE; image courtesy of Haining Zhang, University of Wisconsin-Madison).

For investigation of the genetic basis of a mutation, sequences of proteins and genes are made accessible online. Tools for sequence alignment are linked, giving students the opportunity to align different genomic sequences of these embryos in order to identify specific sites in the genes and protein that lead to the mutant defect. For instance, students could identify the specific genes of interest by taking these sequences and running a query using tools such as BLAST in the database WormBase.org.

Peer Persuasion.

Peer persuasion can occur in various stages during student inquiry using the above-mentioned materials. It provides students the opportunity to share with their peers their observations, hypotheses, and conclusions. For example, many students believe that an embryo increases in volume during early development (our unpublished data). By observing the wild-type embryo development of C. elegans, students may be surprised to find that the embryo does not increase in volume. A student who observes just a single optical plane over time may come to the conclusion that the embryo does increase slightly in volume during early development, whereas a second student, who more carefully observe all optical sections over time, may come to the conclusion that there is no change in volume. During peer persuasion, other students may find that the first student's case is not persuasive, as the student did not do a thorough investigation of all sections over time. Through discussion, the group of students may conclude the embryo of C. elegans does stay constant in volume during early development due to egg shell confinement, or this discussion may initiate further inquiry into more precise measurements of the embryo volume. The peer-persuasion approach can be built into a curriculum in various ways, such as midcourse and final presentations, or by having students present their work in local conferences that are related to topics under their investigation. It provides students, as it does for scientists, the opportunity to reflect on their work as they defend their findings and further refine their work if needed.

In summary, we have illustrated the possible use of C. elegans research resources for students learning by inquiry. Other applications of these resources include classroom demonstration of a phenomenon or developing question sets for student investigation.

For classroom use of WormClassroom site, instructors will drive the materials selection process and how to best incorporate it into their curriculum. Instructors may find it valuable to start with "About C. elegans." This orients instructors to C. elegans as a model organism, and provides examples of research. "C. elegans Education" includes teaching examples titled "a survey of currently used C. elegans curricula" by educators in the C. elegans field. It includes teaching examples ranging from introductory to advanced courses such as genetics and molecular biology in lab and lecture formats. Examples presented would inspire instructors in thinking about the use of C. elegans in the classroom to further develop their own C. elegans curricula. Contributed instructional materials are available right below the surveyed C. elegans curricula categorized into disciplines with different grade levels. These teaching materials include lab protocols, materials for lecture, and inquiry learning modules. For instructors interested in C. elegans for teaching a particular discipline, the links to individual disciplines will acquaint instructors with the use of C. elegans in a specific research field followed by links to the contributed instructional materials page mentioned. In addition to the designated instructional materials page, many C. elegans research datasets are available within the site that instructors may also find useful.

Assessment of the WormClassroom Website

Because there is no established set of assessment criteria for the application of research resources to inquiry learning, we developed our own criteria for assessing areas of the WormClassroom site that would benefit from evaluation. Since we aim to enhance student learning by using research resources of C. elegans, we have focused our primary assessment in three major areas: the assessment of 1) scientific content by experts, 2) website usability, and 3) student learning using resources from the WormClassroom. The assessment included the participation of students and professionals, such as biologists and website developers. Nonstudent participants are referred to as experts in our discussion below.

Assessment of Scientific Content by Experts.

The major focus of the assessment included the following questions: 1) Is the information up-to-date and scientifically correct? 2) Does the scientific content introduce important concepts for further study? and 3) Is the content information adequate for the target audience? (see Table 3). We invited biologists and instructors teaching introductory biology to comment on the content of the WormClassroom website. With the collected feedback from all the experts, corrections were made to address inaccuracies.

Nielsen (2003) argued that users leave a website if it is not easy to navigate. Because our resources are online, assessment of website usability is vital. By testing the website usability, we intended to understand 1) if the site effectively conveys its information to the user and 2) if the interface elements were usable.

For the Web usability study (see Table 4), a pilot testing was performed to try out the test procedure (Nielsen, 1993, 2000). We then recruited five undergraduate students ranging from freshmen to senior, with backgrounds in biology, for the study. To understand the usability of the site, students were encouraged to browse the website freely and spend as much time as they wanted on what interested them. Students were asked to "think out loud" to express their thoughts, opinions, and feelings about the WormClassroom site as they explored it. The same facilitator was with the participants all the time to remind them to express their thoughts on what was visited. In addition, the facilitator helped solve any technology problems that arose. Student interaction was recorded using SnapZ X (Ambrosia, 2005) running on the same computer to record the vocal comments as well as the cursor movement through the entire sitting. Recording student interaction with the website provided insight into why students did what they did, what interested them, and what message was transferred to them. In addition to testing by students, we have also gathered comments from experts such as e-learning consultants, instructional technologists, Web developers, educators, and worm researchers for suggestions on Web usability improvement.

The purpose of this assessment is to understand student learning using C. elegans resources (see Table 5). We developed a lesson using materials from the WormClassroom website. We worked closely with students, collected their written work, and videotaped their learning using the materials. The details of this study will not be described here because it requires lengthy discussion (Lu, Eliceiri, White, and Stewart, unpublished data).

We completed the assessment and revision of scientific content before the website usability testing. This way, the usability assessment could be more focused on questions we intended to answer and avoided the distraction of incorrect scientific information. The scientific content assessment is often easier than the other areas to revise because it usually reveals specific items that need to be corrected. The other two types of assessment may be more difficult to analyze because they can yield input that is more general in nature, such as trying to make the interface more "user friendly." The scientific content assessment was undertaken by a curriculum developer with Web skills, working closely with biologists going page-by-page through the whole site for content accuracy. With the assessment questions in mind, the correction of the content can be updated immediately. Any uncertainty was brought to other experts for later correction or clarification. With scientific discoveries being made daily, the content will be updated followed by a regular content assessment. When adding future resources, we plan to invite C. elegans educators specialized in different disciplines as facilitators to ensure the quality of the resources.

For Web usability assessment, the collected data were QuickTime movies recording students' voices and cursor movements. The data were transcribed and analyzed to examine major themes and patterns in students' browsing the Web site. Some example findings are listed in Table 6. Insights were categorized into issues regarding the content, interface, and animations. For instance, in the "interface issues" category, we found that image links, although popular in current Web design, are confusing to students. Students are often drawn to images as opposed to simple text links. With prevalent use of image links in current Web design, students (with some Web experience) learned they can click on images to see either enlarged images or access pages about these images. However, not all images are links. This often caused frustration during Web browsing once students found they got nowhere by clicking on nonlink images. More importantly, image links with valuable information may be overlooked if the users did not mouse-over them.

We expected that site content and website usability would be major factors in attracting and retaining student interest. What came as some surprise to us was the impact of other attributes of C. elegans research in getting student attention. Examples of such attributes include anecdotes from the research community and research honors such as the 2002 Nobel Prize awarded to researchers studying C. elegans. Understanding what is of most interest to students can be used to incorporate more interesting resources for student learning that can serve to motivate them to use the materials.

	CONCLUSION AND DISCUSSION

TOP ABSTRACT INTRODUCTION C. elegans: THE ORGANISM... THE EDUCATIONAL PORTAL: THE... CONCLUSION AND DISCUSSION REFERENCES

 
The goal of WormClassroom website development is to aid student learning using research resources of C. elegans. As stated in BIO2010 (NRC, 2003), the development of teaching materials such as WormClassroom is a significant undertaking that requires not just time but expertise both in the subject matter and pedagogical approach. Motivated from our desire to share C. elegans resources with the undergraduate biology education community, we utilized the 3 P's pedagogical approach in combination with the multidisciplinary nature of C. elegans research and the consensus practice among scientists in the same research community for our resources application to education. WormClassroom is a project that will evolve with feedback gathered from iterative assessment of both scientific content and student learning. Resources in the WormClassroom website are made accessible in a format designed to promote student learning by inquiry. Assessment was performed to make certain that the content is scientifically correct and that the Web interface is usable. Findings from the assessments were then used to guide us in improving WormClassroom. Student learning using materials in the WormClassroom website has been collected and is under analysis for insight on the effectiveness of C. elegans research resources in student learning.

Despite its primary target of undergraduate biology education, resources in the WormClassroom site are not limited to undergraduate use. We encourage and welcome materials sharing for various levels of education in the WormClassroom. We have attempted to provide as many resources as possible while still leaving instructors the freedom to determine learning objectives when using the resources. Although the resources were developed with inquiry learning in mind, they can also be used to demonstrate phenomena or to develop question sets. Feedback from instructors indicates that they preferred having specific lesson plans available in WormClasssroom in addition to the general IMs and resources. These lesson plans are geared toward a specific course level and have clear daily activities identified with corresponding learning objectives. The hope is that instructors can share lessons and fine tune the various lesson plans to fit the particular needs of their courses. As a result, in addition to a collection of research resources, specific lesson plans for inquiry may be another way to encourage instructors to incorporate research resources to aid student learning.

The major resources in the current version of the WormClassroom site are from our lab, other C. elegans educators and researchers interested in teaching, and online C. elegans research websites. We have also invited C. elegans researchers specializing in various fields of study to contribute resources. Although researchers were enthusiastic regarding our efforts, time constraints often limited participation. Ideally contributions by researchers would be counted as a positive factor toward one's academic career. The Boyer Commission on Educating Undergraduates in the Research Universities (Boyer, 1998) has called for the faculty reward structure to be reconsidered at research universities to specifically reward efforts made in improving teaching. Contributions to an online resource for education, such as WormClassroom, could be viewed as enhancing one's teaching contribution and hopefully be counted toward one's academic credits.

Visibility and maintenance of the WormClassroom are two additional pressing issues for building an education resource of C. elegans for inquiry learning. The two issues are interdependent. For WormClassroom to be successful, it must be current and accurate. The best way to ensure this is widespread use and participation. To increase its visibility, we have submitted the WormClassroom site to the major C. elegans research website lists such as WormBase, WormAtlas, the C. elegans WWW server, and the biology Wikipedia under C. elegans (Wikipedia, 2005). We have also submitted our work to leading education sites such as BioQUEST (2005) and MERLOT (2003). As for maintenance, often one may encounter dead links or out-of-date information while surfing online. It is a challenge to keep a website up-to-date without considerable effort. Another challenge includes finding ways to ease the process of material contributions to the site. It is important to streamline the submission process without a loss in quality of the contributed materials. Additional efforts need to be invested to automate as much of the site administration as possible and yet still include peer review as an important component.

Future Direction

Our intent is to develop a C. elegans education resource based on WormClassroom that may act as a counterpart to the C. elegans research resource WormBase (WormBase, 2000). To sustain our endeavor, more efforts need to be invested in different stages of the project including research resources, funding resources, and human resources. We are the initiator and the major contributor to the development of the WormClassroom website. However, for its further development, contributions are needed from diverse professionals. Funding is critical to sustain its development as well. The National Science Foundation does have funding for integration between research and education, such as the Arctic Research and Education Program (National Science Foundation, 2005). We hope our effort in applying resources of C. elegans to enhance student learning by inquiry, as well as similar efforts by other groups, will be recognized and supported by funding agencies to ensure their sustainability.

In summary, we developed WormClassroom for the utilization of C. elegans resources to aid student learning. We hope others will find it valuable and join our effort. Our ultimate goal is to form an education community of C. elegans based on WormClassroom to aid the current biology education reform effort.

	ACKNOWLEDGMENTS

 
The authors acknowledge all our project contributors listed at http://www.wormclassroom.org/contacts.html who helped make the development of WormClassroom.org possible. We thank Dr. John Jungck of Beloit College for his useful comments on this manuscript. We also thank members of the Spring 2006 Science Education seminar group at the University of Wisconsin-Madison. This work was supported under National Science Foundation Grant DBI-9983114 and National Institutes of Health, National Institute of Biomedical Imaging and Bioengineering Grant R01-EB000184.

	FOOTNOTES

 
Address correspondence to: Kevin W. Eliceiri (eliceiri{at}wisc.edu).

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