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Cell division in human cells. Human epithelial (HeLa) cells were fluorescently labeled for microtubules (green), kinetochores (pink/red), and DNA (blue). Digital images were collected with a Yokogawa CSU-10 spinning Nipkow disk confocal and Hamamatsu ORCA-ER cooled CCD camera mounted on a Nikon TE2000 inverted microscope. The images were then deconvolved, pseudo-colored, and merged using MetaMorph software. Three metaphase mitotic cells are visible with condensed chromosomes aligned at the bipolar spindle equator. A late telophase cell undergoing cytokinesis is also present. Image courtesy of Adrian Salic and Jennifer Waters Shuler, Nikon Imaging Center at Harvard Medical School, Dept. of Cell Biol., 240 Longwood Ave., Boston, MA 02115. E-mail: jshuler@hms.harvard.edu.
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