We acknowledge Dr. Niki Robertson and her laboratory (NCSU), the Caenorhabditis Genetics Center (University of Minnesota), Tranzyme Pharma (Durham, NC), and the Morimoto laboratory (Northwestern University, Evanston, IL) for organisms and reagents. We thank our lab manager, Melissa Cox, for time in ordering and preparing reagents for the laboratories. We also thank the students for effort and willingness to participate in this study. Finally, we thank the NCSU Biotechnology Program for support of this course.