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pClone: Synthetic Biology Tool Makes Promoter Research Accessible to Beginning Biology Students

A. Malcolm Campbell

Address correspondence to: A. Malcolm Campbell (E-mail Address: [email protected]).

*Department of Biology, Davidson College, Davidson, NC 28035

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Department of Biology, Missouri Western State University, St. Joseph, MO 64507

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Department of Psychology, Missouri Western State University, St. Joseph, MO 64507

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Corinne Andresen

Department of Biology, Missouri Western State University, St. Joseph, MO 64507

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Department of Biology, Missouri Western State University, St. Joseph, MO 64507

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Samantha Huckuntod

Department of Biology, Missouri Western State University, St. Joseph, MO 64507

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Claire Shinneman

Department of Biology, Missouri Western State University, St. Joseph, MO 64507

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*Department of Biology, Davidson College, Davidson, NC 28035

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Department of Biology, Missouri Western State University, St. Joseph, MO 64507

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Mary Lee Ledbetter, Monitoring Editor

Published Online:13 Oct 2017https://doi.org/10.1187/cbe.13-09-0189

Supplemental Material

combinedsupmats1.pdf (1 MB)

Vol. 13, No. 2

June 01, 2014

159-359

Supplemental Materials

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Downloads & Citations

Downloads: 329

Citations: 16

History

Submitted: 21 September 2013

Revised: 18 January 2014

Accepted: 18 January 2014

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Information

© 2014 A. M. Campbell et al. CBE—Life Sciences Education © 2014 The American Society for Cell Biology. This article is distributed by The American Society for Cell Biology under license from the author(s). It is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

ACKNOWLEDGMENTS

We thank the students of the Fall 2012 Biology 113 class at Davidson College and the Fall 2013 and Spring 2013 Biology 205 classes at MWSU for test-driving the pClone system and participating in educational research. Thanks to the Fall 2013 Biology 205 class at MWSU for designing and cloning the promoters in Supplemental Figure S2. We also thank Drs. Mark Barsoum and Dave Ashley for granting us access to their students for use as comparisons and Dr. Steve Hatch for the suggestion of using ImageJ. Support is gratefully acknowledged for National Science Foundation RUI grants MCB-1120578 to Davidson College and MCB-1120558 to MWSU; Howard Hughes Medical Institute grants 52005120 and 52006292 to Davidson College; the Davidson Research Institute and James G. Martin Genomics Program at Davidson College; and the MWSU Program of Research, Teaching, and Applied Learning (PORTAL). A.M.C. and T. E. are members of GCAT SynBio.