This work was carried out with support from the National Science Foundation’s grant for MOSART HS LS (EHR-1316645). Any opinions, findings, and conclusions in this article are the authors’ and do not necessarily reflect the views of the National Science Foundation. We thank the 70 scientists who reviewed and commented on the items in the development process. In addition to the authors, the project staff contributing to this research project comprised Annette Trenga, who handled data input and tracking of test forms; John Murray, who programmed and administered online tests; and Harold Coyle, who managed the project. Content consultants who developed items included Joel Mintzes (California State University, Chico) and Kimberly Tanner (San Francisco State University). Jimmy de la Torre (Hong Kong University) provided psychometric support. Andrea Kotula carried out reading-level analysis. Kerry Charles created technical illustrations. Horizon Research provided external evaluation. Our advisory board consisted of Michael Edgar (Milton Academy), Noel Michele Holbrook (Harvard University), Michael Klymkowsky (University of Colorado at Boulder), and Barbara Speziale (Clemson University). We appreciate the advice and support of our colleagues Charles Alcock and Wendy Berland of the Center for Astrophysics | Harvard & Smithsonian. Last but not least, we are grateful for the participation of the many high school life science teachers and their students, without whom this research would have been impossible.